What Is an Oligo Pool? Design, Synthesis, QC & Vendor Decisions
An oligo pool is a mixed library of many DNA oligonucleotides synthesized together for high-throughput workflows such as CRISPR screens, custom NGS panels, gene assembly, and mutagenesis. Start here to understand the concept, then move into design rules, synthesis choices, QC interpretation, vendor short-listing, ordering checks, and application-specific workflows.
Oligo Pool Design & Ordering
Design an Oligo Pool Before Ordering
Move from application choice to order-ready sequences with a five-step pre-order checklist.
Check Oligo Pool Design Rules
Check length limits, GC content, homopolymer runs, and other sequence rules before ordering.
Order an Oligo Pool
Prepare file formats, naming rules, pool-size checks, and a submission checklist before ordering.
Estimate Oligo Pool Cost
Estimate pool cost by application, length tier, QC package, and vendor add-ons before requesting quotes.
Oligo Pool Applications
Design a CRISPR sgRNA Oligo Pool
Size genome-wide or focused sgRNA libraries, run pre-order QC, and choose the right vendor before ordering.
Assemble Synthetic Genes from an Oligo Pool
Plan fragment lengths, overlap design, and assembly steps before ordering a gene synthesis pool.
Design a Custom NGS Panel Oligo Pool
Plan hybrid capture or amplicon probes with panel sizing, tiling, and QC checks before ordering.
Design a Mutagenesis Oligo Pool
Plan DMS and saturation libraries with explicit variants, balanced coverage, and sequencing-ready QC.
Oligo Pool QC, Vendors & Troubleshooting
Read Oligo Pool QC Metrics
Interpret uniformity, dropout, error rate, Gini, and representation metrics in vendor or NGS QC reports.
Choose Between Array and Column Synthesis
Compare array-based and column-based synthesis before you choose a vendor or workflow.
Troubleshoot Oligo Pool Problems
Diagnose dropout, skew, low accuracy, and cloning failures before you reorder.
Compare Oligo Pool Vendors
Compare Twist, IDT, Agilent, and GenScript by pool size, max length, QC, and turnaround.
Oligo Pool Evidence & Decision Pages
Use these pages when the main guide is no longer enough and you need source-backed QC bands, public vendor specs, or final submission checks.
QC Threshold Evidence
Source-backed bands for CRISPR, DMS, MPRA, and gene assembly pools.
Vendor Specs Snapshot
Public length, capacity, turnaround, QC wording, and ordering caveats.
Vendor Decision Page
Turn public specs into a shortlist before requesting quotes.
Ordering Checklist
Check file formats, IDs, pool size, and final submission details.
Ready to Move From Planning to Vendor Review?
Use the vendor comparison and ordering checklist after your design constraints, QC expectations, and pool format are clear.