Wet-lab / NGS Validation
CRISPR Library Experiment Planner
Estimate guide representation, MOI-adjusted seed cells, replicate scale, controls, and selection bottleneck before screening.
Workflow role: run this step inside the design, QC, representation, and vendor-prep chain rather than treating one result as order approval.
Boundary: This is a scale and representation planner. It does not choose guide sequences, score off-targets, model biology, or replace screen-specific experimental review.
Privacy: Calculations run in your browser by default. Dashboard History can store the full input snapshot when auto-save, session, or account history is enabled; Library saves and membership/API workflows can send sequence assets by design.
Handoff: use the Oligo Pool Guide, Batch QC, Vendor Adapter, and QC templates after results.
Input
Use the starter rows to see the input shape, then replace them with your own design rows.
Results
Method, Example, and Limits
Method basis
- Guide-count, MOI, cells-per-guide, replicate, and control-ratio arithmetic.
- Representation and bottleneck checks for pooled screen planning.
- Scale estimates for transduced cells and seed-cell requirements.
Example input
80,000 guides, 500 cells per guide, MOI 0.3, two replicates, and a control-guide percentage.
How to read the result
Use the bottleneck and representation flags to decide whether scale, replicate count, or guide count needs adjustment before ordering.
Not a substitute for
It does not pick sgRNA sequences, evaluate off-target risk, or model screen biology.