About OligoPool.com

Free browser-based tools for oligonucleotide design, primer review, and oligo pool quality checks.

Mission

OligoPool.com provides free calculation tools for oligonucleotide design and analysis. The method pages document the published parameters and assumptions used by the calculators.

The site focuses on common research tasks: melting-temperature checks, primer QC, GC analysis, secondary-structure review, dilution planning, batch sequence QC, and vendor-file preparation.

Core Principles

100% Free

No paywalls, subscriptions, or required accounts for the public calculators.

Privacy First

Calculations run in the browser. Sequence text, uploaded files, and result rows are not sent to the server for analysis. Basic site analytics do not collect sequence content or calculation results.

Scientifically Accurate

Methods based on published literature (SantaLucia 1998, Owczarzy 2008, etc.). Method assumptions are documented so calculated values can be checked against the intended use case.

Open & Transparent

Calculation methods are documented, and bug reports or method questions can be sent directly through the contact form.

Technical Implementation

Built with modern web technologies: Next.js, React, TypeScript, Tailwind CSS. Static site generation ensures fast loading and works offline after initial visit. All calculations implemented in client-side TypeScript—no backend required.

Hosted on Cloudflare Pages for global CDN distribution. We use Google Analytics for basic website usage statistics (anonymized, no personal data). All calculation code is client-side JavaScript—no backend processing of your sequences.

Editorial & Method Governance

How the site is organized

The site separates broad pool planning, synthesis planning, vendor short-listing, and method validation so each page has a clear job. Start with /oligo-pools, use /guides/oligo-pool-synthesis, compare vendors at /oligo-pools/vendor-comparison, and review method notes in /research.

Method and vendor notes

Vendor-sensitive and method-sensitive pages include visible update dates, source notes, and links to references where practical.

What we publish vs what we do not

OligoPool.com is a decision-support and tool site. It does not present itself as a synthesis vendor, a live quote engine, or a regulated lab service provider. Where claims need evidence, we point to References and Accuracy Validation.

How to read our technical pages

Open the linked source notes when a result depends on vendor limits, validation conditions, or published thermodynamic assumptions.

Limitations & Transparency

This is a tool website, not a comprehensive laboratory software suite. Products like Primer3, OligoAnalyzer, or Geneious offer more features. I don't claim to replace them.

Support is limited. Bug fixes and method corrections take priority over new features.

Validate critical designs independently. Calculated values should be checked against the assay context, vendor requirements, and experimental controls.

Future Development

Planned improvements (no timeline promises):

  • Mobile app (offline-capable PWA)
  • Bulk sequence library management
  • Advanced visualization options
  • Export to vendor order formats

Development driven by user feedback and my available time. Open to contributions if this becomes an open-source project in the future.

Get in Touch

Questions, bug reports, or suggestions? I read every message.