For Biotech Companies
Biotech Oligo Design Tools for CRISPR, QC and Orders
Browser-based tools for CRISPR library design, oligo pool QC, and vendor order preparation. Use them for early review, ordering checks, and team review files with browser-default calculations; optional saves follow the shared History, Library, membership, and API data boundary.
Use this page to connect project tasks with the right calculator: batch QC, coverage sizing, vendor-format export, GC balance analysis, structure checks, synthesis-risk modeling, and dilution planning.
Why Biotech Teams Use OligoPool
No IT Overhead
Browser-based tools require no local installation and avoid dependency conflicts. New team members can open the same calculators from a standard web browser.
Data Security by Design
Calculations run in your browser by default. Dashboard History can store the full input snapshot when auto-save, session, or account history is enabled; Library saves and membership/API workflows can send sequence assets by design. Teams can decide which optional saves become reusable backend records.
Scale Without Licensing
Process 1,000 sequences free or 50,000 sequences with Tool Membership per batch analysis. Your team can use the browser tools without per-seat setup.
Review-Ready Outputs
Export results as CSV and generate vendor-specific order files for IDT, Twist, GenScript, and other ordering paths.
Biotech Project Tasks
Tools organized by the sequence review stage.
CRISPR Screen Library Design & Validation
Design and validate genome-scale sgRNA libraries before synthesis. Ensure every guide is synthesis-compatible, free of problematic structures, and meets uniformity requirements for powered screens.
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Catch synthesis-risk guides before ordering and reduce manual redesign loops.
Targeted Panel & Capture Probe Design
Design hybridization capture probes or targeted sequencing panels. Optimize Tm uniformity across all probes for even capture efficiency.
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Identify Tm outliers before panel review so capture probes can be adjusted before ordering.
Gene Synthesis & Assembly
Design overlapping oligos for Gibson assembly or gene synthesis. Verify overlap Tm, check for mispriming, and prepare vendor orders.
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Predict synthesis quality before ordering and plan appropriate purification strategies.
Vendor Integration
Generate order-ready files for your preferred synthesis vendor.
IDT oPools
Excel/CSV export ready
Twist Bioscience
Excel/CSV export ready
GenScript
Excel/CSV export ready
Dynegene
Excel/CSV export ready
Accelerate Oligo Design and Review
Free tools your entire team can use today. No procurement process needed.