Guides & Learning Resources
From beginner concepts to advanced workflows — everything you need to design better oligonucleotides.
In-Depth Guides
PCR Primer Design & Validation
Complete guide to designing effective PCR primers: Tm optimization, GC content, secondary structure checks, and troubleshooting.
Oligo Pool Synthesis Guide
Everything you need to know about ordering and validating oligo pools: vendor selection, QC workflows, and library design.
Learn
Understanding GC Content
What GC content means for your oligos, why it matters for synthesis and PCR, and how to interpret GC distribution.
IntermediateDNA Secondary Structures Explained
Hairpins, self-dimers, G-quadruplexes, and i-motifs. How they form, why they matter, and how to predict them.
IntermediateOligonucleotide Synthesis Methods
Column-based vs array-based synthesis. Phosphoramidite chemistry explained. How synthesis method affects quality.
Solutions by Audience
For Academic Labs
Free tools with no login — built for grad students, postdocs, and PIs.
For Biotech Companies
Scale from target discovery to assay development with zero IT overhead.
For Pharma R&D
Pharma-grade data privacy. Reproducible calculations for documentation.