Lab workflows

Oligo Design Tools by Lab Type

Choose the audience path that matches the work in front of you, then move into the calculator, sequence QC, vendor file, or oligo pool planning page that owns the actual task.

Choose an Audience Path

These pages organize common lab situations without replacing the calculator or oligo pool workflow pages.

Academic labs

Free primer, dilution, CRISPR coverage, and batch sequence QC workflows for research and teaching labs.

Biotech teams

Browser-default tools for CRISPR libraries, targeted panels, vendor files, and repeatable pre-order checks.

Pharma R&D

Sequence checks for target validation, qPCR assay work, ASO review, vendor management, and study documentation.

Choose by the Job to Be Done

If the lab type is less important than the immediate task, start here.

Task	Start Page	Use When	Next Check
Check a few primers before ordering	Tm Calculator	You already have primer sequences and need method-based Tm, salt assumptions, and batch export.	Move to Primer Analyzer if dimer, hairpin, mismatch, or BLAST handoff matters.
Review a mixed sequence set	Batch Sequence QC	You need length, GC, Tm, homopolymer, duplicate, and vendor-risk checks across many oligos.	Use Vendor Format Adapter after failed rows are resolved.
Plan an oligo pool order	Oligo Pool Workflow	The decision includes pool size, synthesis vendor, QC expectations, and order-file preparation.	Compare public vendor specs before requesting quotes.
Prepare a vendor upload file	Vendor Format Adapter	The sequence list is ready and the next step is a vendor-specific CSV or spreadsheet.	Confirm current portal columns and quote terms with the vendor.

Open the Primary Workflow

Start with the page that produces the result or decision, then use the audience pages for context.

Start with the Oligo Pool workflowUse this route when the job includes pool design, synthesis choices, QC, vendors, and ordering decisions.

Calculate primer TmOpen the Tm Calculator for matched salt settings, batch primer checks, and method-based melting temperature results.

Run all-in-one primer reviewUse the Primer Analyzer for Tm, GC%, hairpin, dimer, mismatch, and BLAST handoff checks in one workspace.

Screen a sequence poolUse Batch Sequence QC to review 1,000 free or 50,000 member oligos for GC, Tm, length, homopolymers, and sequence risk.

Size CRISPR coverageOpen the Coverage Calculator for screen representation, tiling libraries, and custom oligo pool sizing.

Prepare vendor order filesUse Vendor Format Adapter when sequence data is ready and the order needs vendor-specific columns.

Prepare stocks and dilutionsUse the Dilution Calculator for resuspension volumes, working stocks, pool dilutions, and printable prep steps.