Oligo Vendor Format Converter - IDT, Twist, GenScript & Dynegene

Convert FASTA, CSV, or plain text sequences to vendor-specific order formats for IDT oPools,Twist Bioscience, GenScript, and Dynegene. Automatically validate sequences, calculate GC content, and export Excel/CSV files ready for direct upload to vendor ordering systems. Updated for 2026 vendor specifications.

Batch processing: 10,000 sequencesClient-side: Complete data privacyFree tool: No registration required

Input Sequences

Accepts FASTA, CSV, or plain sequences. Max 10,000 sequences.

Naming Options

Enter sequences to see results

Converted data will appear here

Why You Need a Vendor Format Adapter

Each oligonucleotide synthesis vendor has its own proprietary ordering format — different column names, required fields, scale options, purification codes, and file formats. Converting your sequences from a universal format (FASTA, CSV) into vendor-specific order files is tedious and error-prone when done manually, especially for large oligo pools with thousands of sequences.

Our Vendor Format Adapter supports the four major synthesis platforms: IDT (oPools and standard oligo plates), Twist Bioscience (oligo pools and gene fragments), GenScript (individual oligos and pools), and Dynegene (custom chip synthesis). The tool auto-generates vendor-compliant Excel/CSV files with proper column headers, scale options, purification methods, and sequence validation — ready for direct upload to the vendor's ordering portal.

Beyond simple format conversion, the adapter performs pre-submission validation: checking sequence length against vendor limits, flagging sequences with characters not accepted by the vendor, calculating GC content for synthesis feasibility assessment, and verifying that pool sizes fall within the vendor's supported ranges. This catches ordering errors before they reach the vendor and avoids costly delays.

How to Use the Vendor Format Adapter

  1. Upload your sequences: paste from clipboard, upload a FASTA/CSV file, or paste tab-separated name-sequence pairs.
  2. Select the target vendor: IDT, Twist Bioscience, GenScript, or Dynegene.
  3. Configure order options: synthesis scale (25 nmol, 100 nmol, etc.), purification method (Desalting, HPLC, PAGE), and naming scheme.
  4. Review the validation results: any sequences that violate vendor limits (length, characters, pool size) are flagged.
  5. Download the formatted Excel or CSV file — it's ready for direct upload to the vendor's ordering portal.

Frequently Asked Questions

What format does IDT require for oligo pool orders?
IDT oPools require a CSV or Excel file with columns: Pool Name, Sequence Name, Sequence (5' to 3'). Additional optional columns include Scale (typically "oPool" for pools), and Notes. Sequences must be 15-350 nt for oPools (standard) or 15-200 nt for oPools with modifications. IDT individual plates require: Well Position, Name, Sequence, Scale (25nm/100nm/250nm/1um/10um), Purification (STD/HPLC/PAGE). Our adapter handles both formats.
What are the sequence length limits for each vendor?
IDT oPools: 15-350 nt. Twist Oligo Pools: 15-300 nt (standard), up to 350 nt with extended Oligo Pools. GenScript standard oligos: 5-100 nt. GenScript gene fragments: 100-3000 bp. Dynegene chip synthesis: 20-230 nt. Our adapter validates against these limits and flags any out-of-range sequences. Note that maximum length may vary based on specific product lines and current vendor capabilities.
How do I choose between vendors for my oligo pool?
Key factors: (1) Pool size — Twist and IDT handle up to 100,000+ sequences, while some vendors have lower limits; (2) Sequence length — Twist offers up to 300 nt standard, IDT oPools up to 350 nt; (3) Quantity — IDT oPools deliver 2-10 pmol/oligo, Twist delivers 25-100 fmol/oligo; (4) Uniformity — both Twist and IDT achieve <3× fold-difference for standard pools; (5) Turnaround time — typically 2-4 weeks. Visit our Vendors page for a detailed comparison.
Can I include modifications in my vendor order?
Yes, but modification support varies by vendor and product line. For IDT: standard modifications (5'-phosphate, biotin, fluorophores) are supported for individual oligos but not for oPools. For Twist: oligo pools are unmodified only. For GenScript: extensive modification catalog for individual oligos. Our adapter includes modification fields where supported and warns when modifications are not available for the selected product type.
What happens if my sequence contains invalid characters?
Our adapter validates all sequences against the target vendor's accepted alphabet. Most vendors accept standard DNA bases (A, T, C, G) and some accept IUPAC ambiguity codes (R, Y, S, W, etc.) depending on the product type. Sequences containing invalid characters (numbers, spaces, non-IUPAC letters) are flagged with specific error messages. You can fix these in the tool before downloading the order file, preventing submission errors and order delays.

Related Tools

Format Converter

Convert between FASTA, CSV, TSV formats before using the Vendor Format Adapter.

Batch Sequence QC

Validate all sequences for synthesis compatibility before generating vendor order files.

Uniformity Estimator

Predict expected uniformity and dropout rate for your chosen synthesis platform.

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Synthesis Vendors ComparisonPre-Order Oligo Pool QCUser Guide