All Tools
Comprehensive suite of free online tools for oligonucleotide design, analysis, and quality control. All calculations run in your browser — your data never leaves your device. User Guide
Basic Calculators
Essential calculations for oligonucleotide design and handling
Oligonucleotide Properties Calculator
Comprehensive all-in-one calculator: molecular weight, GC content, Tm, OD260, reverse complement, and thermodynamic constants.
Tm Calculator
Calculate melting temperature using nearest-neighbor thermodynamics. Supports salt and DMSO corrections.
GC Content Analyzer
Analyze GC percentage with batch processing. Identify extreme compositions and risk levels.
Molecular Weight Calculator
Calculate molecular weight, extinction coefficient, and concentration conversions. Supports modifications.
Dilution Calculator
Calculate resuspension volumes, dilutions, and pool-specific concentrations with printable protocols.
Sequence Analysis
Advanced tools for sequence validation and structure prediction
Secondary Structure Predictor
Predict hairpins, self-dimers, and hetero-dimers. Calculate ΔG for structure stability assessment.
Primer Analyzer
Comprehensive primer analysis including Tm, GC%, self-complementarity, and dimer checks.
Batch Sequence QC
Analyze up to 10,000 sequences for quality issues. Detect homopolymers, low complexity regions, and more.
Quality Control
Tools for assessing oligo pool quality and synthesis parameters
Synthesis Error Rate Calculator
Predict full-length product percentage based on coupling efficiency. Compare with industry standards.
Pool Uniformity Estimator
Estimate dropout rate and concentration variation. Get QC sequencing depth recommendations.
Coverage Calculator
Calculate required oligos for CRISPR libraries, tiling applications, and custom coverage strategies.
Format Conversion
Convert sequences between formats and prepare vendor orders
Why Use OligoPool.com Tools?
100% Free
No fees, no paywalls, no hidden costs. All tools completely free forever.
Privacy First
All calculations run locally in your browser. Your data stays private.
Scientifically Accurate
Based on published methods (SantaLucia 1998, etc.) with proper citations.
Lightning Fast
Instant calculations, batch processing up to 10,000 sequences in seconds.
Need High-Quality Oligo Pools?
For professional oligo pool synthesis services, check out our partner Dynegene. High uniformity, competitive pricing, and fast turnaround.
Learn More About Dynegene