Design / Architecture
Oligo Pool Architecture Builder
Assemble adapters, primers, barcodes, inserts, and restriction motifs into synthesis-ready full constructs before Batch QC.
Workflow role: run this step inside the design, QC, representation, and vendor-prep chain rather than treating one result as order approval.
Boundary: This assembles browser-default construct rows; Batch QC and vendor-specific formatting remain separate review steps before synthesis submission.
Privacy: Calculations run in your browser by default. Dashboard History can store the full input snapshot when auto-save, session, or account history is enabled; Library saves and membership/API workflows can send sequence assets by design.
Handoff: use the Oligo Pool Guide, Batch QC, Vendor Adapter, and QC templates after results.
Input
Use the starter rows to see the input shape, then replace them with your own design rows.
Dashboard Library
Use saved sequences as this tool's input.
Results
Method, Example, and Limits
Method basis
- String assembly from user-provided adapter, barcode, insert, and primer segments.
- Length and motif checks against the selected design constraints.
- CSV/FASTA export for downstream Batch QC and vendor-format preparation.
Example input
Insert rows with optional barcode IDs, a shared 5-prime adapter, a shared 3-prime adapter, and a vendor maximum-length setting.
How to read the result
Review total construct length first, then inspect motif warnings and missing segment flags. Passing rows still need Batch QC before vendor upload.
Not a substitute for
It is not a cloning strategy designer and does not confirm enzyme compatibility, genome uniqueness, or vendor acceptance.