Premium resource
Oligo Pool QC Checklist + Excel Templates
Turn one-off calculator results into a repeatable lab review workflow for batch QC, primer analysis, Tm checks, and secondary-structure risk before vendor upload.
One-time Gumroad purchase, $39. Free calculators stay free.
Template pack
PDF checklist + Excel workbook, delivered by Gumroad
$39
- Batch sequence QC checklist for length, GC, Tm spread, homopolymers, ambiguous bases, and flagged rows
- Primer review worksheet for Tm balance, GC range, hairpin risk, self-dimer risk, and redesign notes
- Secondary-structure risk triage sheet with delta G thresholds and decision labels
- Vendor upload readiness checklist for file formatting, row naming, and final order review
Where it fits
Use this pack after running the free tools, when the next decision is whether a set is ready to order, which rows need redesign, and what needs to be documented for repeat review.
Batch QC
Export length, GC, Tm, homopolymer, and low-complexity flags before writing the review row.
Primer Analyzer
Use the primer report for Tm balance, GC range, molecular weight, and dimer review notes.
Secondary Structure Predictor
Record hairpin, self-dimer, hetero-dimer, and 3-prime endpoint risk labels.
Tm Calculator
Document the method, salt, Mg, dNTP, DMSO, formamide, and concentration assumptions.
Format Converter
Clean FASTA, CSV, TSV, and plain-text rows before the final vendor-format handoff.
Public preview
How the checklist turns calculator output into review decisions
The paid workbook is organized around review categories that also appear in the free tools. The preview below shows the decision logic without requiring a purchase.
| Review category | What the template records | Free path before buying |
|---|---|---|
| Length drift | Flag rows outside the intended construct length before vendor formatting. | Recheck architecture, adapters, and pasted insert boundaries. |
| GC outlier | Separate rows outside the planned GC window from rows that only need routine review. | Move outliers to Primer Analyzer or Batch QC before ordering. |
| Structure risk | Record hairpin, self-dimer, and 3-prime dimer labels in one review column. | Redesign sequences that combine strong structure with endpoint involvement. |
| Vendor handoff | Confirm names, sequences, modification syntax, plate/well fields, and file type. | Use Vendor Format Adapter after QC decisions are settled. |
When this pack is useful
Use it when several people need to review the same oligo pool, when a vendor upload needs a documented pre-check, or when repeated primer and pool decisions should follow the same checklist.
When you should stay with free tools
- You only need a one-time Tm, GC, or structure value and do not need a reusable review record.
- Your lab already has a validated LIMS, ELN, or vendor-specific order-review template.
- You need vendor-side synthesis QC, NGS validation, or empirical wet-lab pass/fail certification.
What you get
A Gumroad-delivered ZIP named oligopool-qc-checklist-template-pack-v1.zip with the PDF checklist, Excel workbook, and README.
How delivery works
The button opens the Gumroad product page. Gumroad handles checkout, receipts, customer library access, and the file download.
What stays free
Batch QC, primer analysis, Tm calculation, and structure prediction remain free tools. The paid pack is for documenting and standardizing the review workflow.
Ready to standardize the review?
Download the QC checklist and Excel template pack
One-time $39 Gumroad purchase. Gumroad delivers the ZIP through the receipt email and library download link.