Design / Architecture
NGS Capture / MPRA Probe Tiling Designer
Tile target regions into probe constructs with adapter schema, avoid motifs, GC checks, and vendor max-length warnings.
Workflow role: run this step inside the design, QC, representation, and vendor-prep chain rather than treating one result as order approval.
Boundary: Probe tiling is a sequence-layout aid. Capture chemistry, empirical enrichment performance, genome uniqueness, and final vendor acceptance need separate review.
Privacy: Calculations run in your browser by default. Dashboard History can store the full input snapshot when auto-save, session, or account history is enabled; Library saves and membership/API workflows can send sequence assets by design.
Handoff: use the Oligo Pool Guide, Batch QC, Vendor Adapter, and QC templates after results.
Input
Use the starter rows to see the input shape, then replace them with your own design rows.
Results
Method, Example, and Limits
Method basis
- Sliding-window tiling from target sequence, probe length, and overlap settings.
- Adapter schema assembly with avoid-motif, GC, and vendor max-length checks.
- Exportable probe rows for Batch QC and vendor-format follow-up.
Example input
A target region, 120 nt probe length, 60 nt overlap, optional adapters, avoid motifs, and a vendor length ceiling.
How to read the result
Review coverage continuity, length warnings, GC outliers, and avoid-motif flags before moving the design to Batch QC.
Not a substitute for
It does not test genome uniqueness, predict capture efficiency, or validate MPRA regulatory activity.