Design / Architecture
Barcode / Index Designer
Generate error-correctable DNA barcodes, check existing index collisions, and export CSV or FASTA for oligo pool tracking.
Workflow role: run this step inside the design, QC, representation, and vendor-prep chain rather than treating one result as order approval.
Boundary: Barcode sets are design aids. Confirm index compatibility with the sequencer, chemistry, demultiplexing settings, and vendor constraints before ordering.
Privacy: Calculations run in your browser by default. Dashboard History can store the full input snapshot when auto-save, session, or account history is enabled; Library saves and membership/API workflows can send sequence assets by design.
Handoff: use the Oligo Pool Guide, Batch QC, Vendor Adapter, and QC templates after results.
Input
Use the starter rows to see the input shape, then replace them with your own design rows.
Keep the same seed for reproducible index sets.
Optional. Lines that look like DNA barcodes are checked for distance collisions.
Results
Method, Example, and Limits
Method basis
- Pairwise Hamming distance screening between generated barcodes.
- GC range and homopolymer-run filters before export.
- Per-position base-diversity summary for index balance review.
Example input
24 barcodes, length 8, minimum Hamming distance 3, 30-70% GC, maximum homopolymer run 3.
How to read the result
Start with the minimum pairwise distance and base-diversity table. Existing-collision issues should be resolved before the barcode set is used in demultiplexing.
Not a substitute for
It does not validate sequencer-specific index recipes, color-balance requirements, or vendor ordering rules.