Parameter Glossary
A comprehensive reference for algorithmic sequence parameters, thermodynamic variables, and synthesis terminology.
PRIMER_MAX_END_GC
The maximum number of Gs or Cs allowed in the last five 3' bases of a left or right primer.
primer3
PRIMER_MIN_TM
Minimum acceptable melting temperature (Celsius) for a primer oligo.
primer3
PRIMER_OPT_TM
Optimum melting temperature (Celsius) for a primer. Primer3 will try to pick primers with melting temperatures close to this temperature.
primer3